BCM-95® (CURCUGREEN®), 28 Mar 2022

doi.org/10.1007/s10522-021-09915-0

Giulia Matacchione, Felicia Gurau, Andrea Silvestrini, Mattia Tiboni, Luca Mancini, Debora Valli, Maria Rita Rippo, Rina Recchioni, Fiorella Marcheselli, Oliana Carnevali, Antonio Domenico Procopio, Luca Casettari, Fabiola Olivieri.

Giulia Matacchione, Felicia Gurău, Andrea Silvestrini, Mattia Tiboni, Luca Mancini, Debora Valli, Maria Rita Rippo, Rina Recchioni, Fiorella Marcheselli, Oliana Carnevali, Antonio Domenico Procopio, Luca Casettari & Fabiola Olivieri

The study investigated both the anti senescence associated secretory phenotype (SASP) and anti-inflammatory activities of a nutritional supplement, namely FenoxidolTM, composed of turmeric extract bioCurcumin (bCUR), Polydatin (the natural glycosylated precursor of Resveratrol-RSV), and liposomal b-caryophyllene (BCP), in two human cellular models, such as the primary endothelial cell line, HUVECs and the monocytic cell line, THP-1. Replicative and Doxorubicin-induced senescent HUVECs, both chosen as cellular models of SASP, and lipopolysaccharides (LPS)-stimulated THP-1, selected as a model of the inflammatory response, were treated with the three single natural compounds or with a combination of them (MIX). In both senescent HUVEC models, MIX treatment significantly reduced IL-1b and IL-6 expression levels and p16ink4a protein, and also increased SIRT1 protein level, as well as downregulated miR-146a and miR-21 expression, two of the so-called inflamma-miRNAs, more effectively than the single compounds. In THP-1 cells stimulated with LPS, the MIX showed a significant effect in decreasing IL-1b, IL-6, TNF-a, and miR-146a expression levels and Caspase-1 activation, in association with an up-regulation of SIRT1 protein, compared to the single compounds. Overall, results suggest that the three analysed compounds can have a combined effect in restraining SASP in senescent HUVECs as well as the inflammatory response in LPS-stimulated THP-1 cells. association on human endothelial and monocytic cells. Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and b caryophylleneBiogerontology, https://doi.org/10.1007/s10522-021-09915-0.]